LOCUS       pLV-[hsa-miR-526a-2]        9158 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7079..7939
                     /label=Amp(R)
     promoter        8140..8680
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5684..5918
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6493..6948)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5990..6120
                     /label=SV40 pA
     misc_feature    6121..6334
                     /label=SV40 ori
     misc_feature    5005..5601
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3623
                     /label=intron
     CDS             3670..4995
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3413
                     /label=hsa-mir-526a-2 precursor
BASE COUNT     2180 a      2356 c      2480 g      2142 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg atgatcctgt 
     3121 gaggggttta tgtttttgga ttcaggaaac acaccagcag gacaaataat tgcatcttta 
     3181 tttttgtgtc tattttcacc aggctaagga agattccaac aacatatcca tggtgctgaa 
     3241 gtaagaagat ctcgtgctgt gaccctctag agggaagcac tttctgttga aagaaaagaa 
     3301 catgcatcct ttcagagggt tactctttga gaagagcaac gtttagtttg atgttgatct 
     3361 tggtagtaca tttgcagagc atgcttttat catcagactt ggctgatggt ggtgctagct 
     3421 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc 
     3481 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct 
     3541 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc 
     3601 aaagtttttt tcttccattt caggtgtcgt gaaaactacc cctctagagt cgagctaccg 
     3661 gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg 
     3721 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 
     3781 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc 
     3841 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac 
     3901 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 
     3961 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 
     4021 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 
     4081 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc 
     4141 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac 
     4201 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc 
     4261 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa 
     4321 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggac 
     4381 ccaccggtcg ccaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 
     4441 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 
     4501 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 
     4561 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 
     4621 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 
     4681 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 
     4741 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 
     4801 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 
     4861 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 
     4921 ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc 
     4981 aagcccggtg cctgagcggc cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa 
     5041 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa 
     5101 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat 
     5161 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt 
     5221 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc 
     5281 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc 
     5341 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg 
     5401 ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga 
     5461 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc 
     5521 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc 
     5581 tttgggccgc ctccccgcct ggaattcgag ctcggtacct ttaagaccaa tgacttacaa 
     5641 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 
     5701 ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 
     5761 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 
     5821 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 
     5881 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 
     5941 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 
     6001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 
     6061 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 
     6121 gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 
     6181 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 
     6241 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca 
     6301 attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg 
     6361 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 
     6421 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 
     6481 atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 
     6541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 
     6601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 
     6661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 
     6721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 
     6781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 
     6841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 
     6901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt 
     6961 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
     7021 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 
     7081 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 
     7141 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 
     7201 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 
     7261 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 
     7321 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 
     7381 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 
     7441 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 
     7501 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 
     7561 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 
     7621 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 
     7681 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 
     7741 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 
     7801 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 
     7861 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 
     7921 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 
     7981 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 
     8041 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 
     8101 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 
     8161 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 
     8221 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 
     8281 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 
     8341 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 
     8401 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 
     8461 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 
     8521 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 
     8581 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 
     8641 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 
     8701 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 
     8761 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 
     8821 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 
     8881 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
     8941 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
     9001 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
     9061 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc 
     9121 aattaaccct cactaaaggg aacaaaagct ggagctgc 
//
